acdtrace Wiki The master copies of EMBOSS documentation are available at http://emboss.open-bio.org/wiki/Appdocs on the EMBOSS Wiki. Please help by correcting and extending the Wiki pages. Function Trace processing of an application ACD file (for testing) Description acdtrace reads and processes an ACD file, prompting the user as necessary in exactly the same way as if the application itself was running. If it encounters an error it will terminate. This allows the behaviour of the command-line interface to be tested under different conditions, even if the application itself has not yet been written. acdtrace works the same as acdc but reports on the processing of lines in an ACD file. It also also prints the results of evaluating any variables and operations in the ACD file. Usage Here is a sample session with acdtrace % acdtrace antigenic Find antigenic sites in proteins Input protein sequence(s): tsw:actb1_takru Minimum length of antigenic region [6]: Output report [actb1_takru.antigenic]: Go to the input files for this example Go to the output files for this example Command line arguments The first parameter is the name of the program to compile. All other command line parameters and qualifiers are defined for that program - see the documentation for the program of choice. Trace processing of an application ACD file (for testing) Version: EMBOSS:6.6.0.0 Standard (Mandatory) qualifiers: [-program] string Program name, followed by command line (Any string) Additional (Optional) qualifiers: (none) Advanced (Unprompted) qualifiers: (none) Associated qualifiers: (none) General qualifiers: -auto boolean Turn off prompts -stdout boolean Write first file to standard output -filter boolean Read first file from standard input, write first file to standard output -options boolean Prompt for standard and additional values -debug boolean Write debug output to program.dbg -verbose boolean Report some/full command line options -help boolean Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose -warning boolean Report warnings -error boolean Report errors -fatal boolean Report fatal errors -die boolean Report dying program messages -version boolean Report version number and exit Input file format The input is whatever the chosen application reads. Input files for usage example 'tsw:actb1_takru' is a sequence entry in the example protein database 'tsw' Database entry: tsw:actb1_takru ID ACTB1_TAKRU Reviewed; 375 AA. AC P68142; P53484; DT 25-OCT-2004, integrated into UniProtKB/Swiss-Prot. DT 25-OCT-2004, sequence version 1. DT 16-MAY-2012, entry version 49. DE RecName: Full=Actin, cytoplasmic 1; DE AltName: Full=Beta-actin A; DE Contains: DE RecName: Full=Actin, cytoplasmic 1, N-terminally processed; GN Name=actba; OS Takifugu rubripes (Japanese pufferfish) (Fugu rubripes). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Euteleostei; Neoteleostei; OC Acanthomorpha; Acanthopterygii; Percomorpha; Tetraodontiformes; OC Tetradontoidea; Tetraodontidae; Takifugu. OX NCBI_TaxID=31033; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY. RX MEDLINE=96275651; PubMed=8683572; DOI=10.1006/jmbi.1996.0347; RA Venkatesh B., Tay B.H., Elgar G., Brenner S.; RT "Isolation, characterization and evolution of nine pufferfish (Fugu RT rubripes) actin genes."; RL J. Mol. Biol. 259:655-665(1996). CC -!- FUNCTION: Actins are highly conserved proteins that are involved CC in various types of cell motility and are ubiquitously expressed CC in all eukaryotic cells. CC -!- SUBUNIT: Polymerization of globular actin (G-actin) leads to a CC structural filament (F-actin) in the form of a two-stranded helix. CC Each actin can bind to 4 others. CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. CC -!- TISSUE SPECIFICITY: Widely distributed. Not expressed in skeletal CC muscle. CC -!- PTM: Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to CC form methionine sulfoxide promotes actin filament CC depolymerization. Methionine sulfoxide is produced CC stereospecifically, but it is not known whether the (S)-S-oxide or CC the (R)-S-oxide is produced (By similarity). CC -!- MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, CC alpha, beta and gamma have been identified. The alpha actins are CC found in muscle tissues and are a major constituent of the CC contractile apparatus. The beta and gamma actins coexist in most CC cell types as components of the cytoskeleton and as mediators of CC internal cell motility. CC -!- MISCELLANEOUS: There are three different beta-cytoplasmic actins CC in Fugu rubripes. CC -!- SIMILARITY: Belongs to the actin family. CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; U37499; AAC59889.1; -; Genomic_DNA. DR PIR; S71124; S71124. DR ProteinModelPortal; P68142; -. DR SMR; P68142; 2-375. DR Ensembl; ENSTRUT00000013141; ENSTRUP00000013080; ENSTRUG00000005447. DR eggNOG; COG5277; -. DR GeneTree; ENSGT00630000089629; -. DR InParanoid; P68142; -. DR OMA; IKNLMER; -. DR OrthoDB; EOG41JZC9; -. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW. DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR InterPro; IPR004000; Actin-like. DR InterPro; IPR020902; Actin/actin-like_CS. DR InterPro; IPR004001; Actin_CS. DR PANTHER; PTHR11937; Actin_like; 1. DR Pfam; PF00022; Actin; 1. DR PRINTS; PR00190; ACTIN. DR SMART; SM00268; ACTIN; 1. DR PROSITE; PS00406; ACTINS_1; 1. DR PROSITE; PS00432; ACTINS_2; 1. DR PROSITE; PS01132; ACTINS_ACT_LIKE; 1. PE 2: Evidence at transcript level; KW Acetylation; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; KW Methylation; Nucleotide-binding; Oxidation; Reference proteome. FT CHAIN 1 375 Actin, cytoplasmic 1. FT /FTId=PRO_0000367094. FT INIT_MET 1 1 Removed; alternate (By similarity). FT CHAIN 2 375 Actin, cytoplasmic 1, N-terminally FT processed. FT /FTId=PRO_0000000809. FT MOD_RES 1 1 N-acetylmethionine; in Actin, cytoplasmic FT 1; alternate (By similarity). FT MOD_RES 2 2 N-acetylglutamate; in Actin, cytoplasmic FT 1, N-terminally processed (By FT similarity). FT MOD_RES 44 44 Methionine sulfoxide (By similarity). FT MOD_RES 73 73 Tele-methylhistidine (By similarity). SQ SEQUENCE 375 AA; 41767 MW; 9C505616D33E9495 CRC64; MEDEIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMGTA ASSSSLEKSY ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTYN SIMKCDVDIR KDLYANTVLS GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF // Output file format The output file is empty, but any format available to the specified program can be used. Output files for usage example File: actb1_takru.antigenic Data files Acdtrace will use any data files specified in the ACD file of the specified program, but will ignore any data files that are only used directly by the program's code. EMBOSS data files are distributed with the application and stored in the standard EMBOSS data directory, which is defined by the EMBOSS environment variable EMBOSS_DATA. To see the available EMBOSS data files, run: % embossdata -showall To fetch one of the data files (for example 'Exxx.dat') into your current directory for you to inspect or modify, run: % embossdata -fetch -file Exxx.dat Users can provide their own data files in their own directories. Project specific files can be put in the current directory, or for tidier directory listings in a subdirectory called ".embossdata". Files for all EMBOSS runs can be put in the user's home directory, or again in a subdirectory called ".embossdata". The directories are searched in the following order: * . (your current directory) * .embossdata (under your current directory) * ~/ (your home directory) * ~/.embossdata Notes acdtrace does not have its own options, but takes a single parameter which is the name of the EMBOSS application that is being testsed. It is invoked by specifying the name of the application to be tested along with any command-line options after the name of the utility application itself: acdtrace ApplicationName Options acdtrace will run the command line interface of any EMBOSS program by reading its ACD file and processing the command line and user responses in exactly the same way as if the true program itself were running. The application proper is not run; it is only the ACD file that is read. Any command-line options for the application being called can be specified. The user is prompted for any required parameter values not given on the command line and any input files are read. Output files can be specified in any available format however they will be empty (the application code is not called so there will be no output). acdtrace will use any data files specified in the ACD file of the specified program, but will ignore any data files that are only used directly by the program's code. EMBOSS data files are distributed with the application and stored in the standard EMBOSS data directory (see EMBOSS Users Manual for further information). References Warnings The output on screen can look a little confusing but is by far the best way to see how variables and operations work in your ACD file. Diagnostic Error Messages Exit status It exits with status 0. Known bugs None. See also Program name Description acdc Test an application ACD file acdpretty Correctly reformat an application ACD file acdtable Generate an HTML table of parameters from an application ACD file acdvalid Validate an application ACD file Author(s) Peter Rice European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK Please report all bugs to the EMBOSS bug team (emboss-bug (c) emboss.open-bio.org) not to the original author. History Target users This program is intended to be used by developers of applications and interfaces. Comments None